Validation of liquid chromatography mass spectrometry (LC-MS) methods

Bonus self test 9.5

1. Estimate CCα and CCβ for LC-MS/MS measurements of triazophos from tomatoes using the method given in ISO 11843-2. Use ValChrom to estimate CCα and CCβ. For those who want to understand the calculation processes behind CCα and CCβ, full calculation logic can be found at [ref 18].

ctriasophos (mg/kg) Parallel 1 Parallel 2 Parallel 3 Parallel 4
0 495 2834 1687 306
8.04E-06 419 981 1777 2141
9.78E-05 3177 6228 2803 4933
5.21E-04 11740 15274 13275 16857
1.21E-03 31638 37982 25181 32632
2.83E-03 60897 60702 60501 63998

 

For use with ValChrom: cca_and_ccb_4_parallels.xls  Use α=β=5% and K=4 during upload.

Write results with 3 significant digits.

Estimation for CCα: (Enter your answer here)

Estimation for CCβ: (Enter your answer here)

2. Estimate LoD from the same given data using ICH method based on calibration graph and residual deviation of residuals.

Write results with 3 significant digits.

(Enter your answer here)

3. Which guideline is more conservative at its estimate of detection capabilities: ICH or ISO?

4. Which guideline makes more assumptions: ICH or ISO?


 

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