Self-test 5.4A – MOOC: Validation of liquid chromatography mass spectrometry (LC-MS) methods (analytical chemistry) course

If blank matrix is not available for the analyst matrix effect can be estimated with:

Comparing calibration graph slopes for pre-extraction spiked samples and standards.

Comparing peak areas for post-extraction spiked samples and standards with the same concentration.

Comparing calibration graph slopes for post-extraction spiked samples and standards.

Process efficiency refers to the joint effect of possible losses during sample preparation and ionization suppression/enhancement in the ion source.

True

False

Process efficiency expresses the efficiency of the sample preparation step.

True

False

You are using a LC-MS (LC is reversed phase) and you observe severe matrix effect. How to remove or reduce matrix effect?

Add solid phase exctraction (C18) procedure for sample concentration to your sample preparation.

Inject smaller sample amount.

Dilute the sample.