Self-test 5.4A – Validation of liquid chromatography mass spectrometry (LC-MS) methods

Process efficiency expresses the efficiency of the sample preparation step.

True

False

If blank matrix is not available for the analyst matrix effect can be estimated with:

Comparing calibration graph slopes for pre-extraction spiked samples and standards.

Comparing peak areas for post-extraction spiked samples and standards with the same concentration.

Comparing calibration graph slopes for post-extraction spiked samples and standards.

You are using a LC-MS (LC is reversed phase) and you observe severe matrix effect. How to remove or reduce matrix effect?

Add solid phase exctraction (C18) procedure for sample concentration to your sample preparation.

Dilute the sample.

Inject smaller sample amount.

Process efficiency refers to the joint effect of possible losses during sample preparation and ionization suppression/enhancement in the ion source.

True

False