Self-test 4.4D – Validation of liquid chromatography mass spectrometry (LC-MS) methods

You are estimating the precision of a method for analysing neonicotinoid acetamiprid in garlic and tomato. Using data from analysis of spiked garlic and tomato samples, evaluate precision of acetamiprid determination on each day and pooled standard deviation of all series.

NB! Consider the significance of intercept and whether calibration line should be forced through 0.

Data for calculations: 44d_garlic_raw.xls and 44d_tomato_raw.xls

Use spreadsheets or ValChrom software (assessment method: Intermediate precision, 6 injections at target concentration).

1. Estimate RSD for tomato samples on 16^th of October. Write results rounded to 3 significant digits (unit: %).

2. Estimate RSD for garlic samples on 16^th of October. Write results rounded to 3 significant digits (unit: %).

3. Estimate pooled RSD for tomato samples over all series. Write results rounded to 3 significant digits (unit: %).

4. Estimate pooled RSD for garlic samples over all series. Write results rounded to 3 significant digits (unit: %).

5. Is the method acceptable for analysis of acetamiprid, if pooled RSD criteria would be 5%?

Yes, for tomato

Yes

No

Yes, for garlic