Self-test 3.4 – Validation of liquid chromatography mass spectrometry (LC-MS) methods

1. Sensitivity can be useful for:

Correcting for matrix suppression

Estimating the trueness of the method

Method optimization

Estimating matrix effect, LoD and LoQ

Deciding if linear calibration model can be used

Routine monitoring of the instrument

2. Find the numerical value and unit of sensitivity of a LC-ESI-MS method for acetamiprid analysis in garlic.

C (ppb)	Signal (au)
0.2	10989337
0.7	20508735
1.5	35998848
2	46888155
3	65076575
4	82250446

For use with ValChrom: st_3.4_1_sensitivity.csv

Sensitivity (please round the answer to nearest integer and choose the correct unit from the given list):

3. Sensitivity is defined as:

Characteristic of method’s detection/determination ability

The change in the response of a measuring instrument divided by the corresponding change in the stimulus

The lower and upper concentrations of analyte in the sample for which precision, accuracy and response function has been established

The slope of the calibration graph

Method’s ability to obtain test results which are directly proportional to the concentration of analyte in the sample