Self-test 3.3 – Validation of liquid chromatography mass spectrometry (LC-MS) methods

1. Linear range is defined as:

The lower and upper concentrations of analyte in the sample for which precision, accuracy and response function has been established

The change in the response of a measuring instrument divided by corresponding change in the stimulus

The range in which the method is able to obtain test results that are directly proportional to the concentration of analyte in the sample

2. Linear range is synonymous to:

analytical range

dynamic range

working range

calibration range

linear dynamic range

3. Linear range of a method with a certain analyte does not depend on the matrix.

True

False

4. Which of the following methods helps to improve the linear range in LC-ESI-MS?

Using isotope-labeled internal standard (ILIS).

Lowering the flow rate in ESI source to reduce the charge competition

Selecting a different fragment ion for quantitation

Changing the order in which the calibration standards are injected

Dilution of the standard solutions and sample solutions