Self-test 3.1 – Validation of liquid chromatography mass spectrometry (LC-MS) methods

1. Linearity is defined as:

The lower and upper concentrations of analyte in the sample for which precision, accuracy and response function has been established

The change in the response of a measuring instrument divided by the corresponding change in the stimulus

Method’s ability to obtain measurement results that are directly proportional to the concentration of analyte in the sample

2. If there is linear relationship between analyte signal and analyte concentration in the calibration samples, there is also most certainly a linear relationship between analyte signal and analyte concentration in the samples containing matrix components.

True

False

3. Which of the following aspects affect linearity of the signal in LC/MS analysis?

Type of ionization source

Type of mass analyzer

Sample matrix

Chosen calibration model

Whether solvent or matrix-matched calibration is used