MOOC: Instrumental analysis of cultural heritage objects

SELF TEST 5.2

Which of the following are soft ionisation techniques? (There can be one or more correct answers)

Chemical ionisation (CI)

Matrix-assisted laser desorption/ionisation (MALDI)

Electrospray ionisation (ESI)

Atmospheric pressure chemical ionisation (APCI)

Electron ionisation (EI)

Which of the following are low-resolution (LR) mass analysers? (There can be one or more correct answers)

Linear Time of Flight (ToF)

Quadrupole (Q)

Ion trap (IT)

Reflectron Time of Flight (ToF)

Fourier Transform Ion Cyclotron Resonance (FT-ICR)

Fourier Transform Orbitrap (FT-OT)

Which of the following applies for MS? (There can be one or more correct answers)

It is a very sensitive technique

It is suitable for qualitative and quantitative analysis

It allows to identify different compounds in a mixture

Different ion sources can be used

Can be combined with chromatography

Several (same or different) mass analysers can be combined (tandem MS)

It separates compounds according to polarity

It detects characteristic molecular vibrations, from which structural information about materials can be obtained

It is suitable for samples of simple composition only

Find the suitable word to complete the following text.

Mass spectrometry is an analytical technique that the created from the of the sample according to their .

Match the ion source with the correct description:

Sample molecules are ionised by bombarding them with high-energy electrons. During this process the analyte ions fragment extensively. The ionisation takes place in vacuum.	APCI Unselect MALDI Unselect CI Unselect ESI Unselect EI Unselect
Sample molecules are ionised by a reagent gas. During this process mostly protonated ions ([M+H]+) are formed. The ionisation takes place in vacuum.	APCI Unselect MALDI Unselect CI Unselect ESI Unselect EI Unselect
Sample solution is sprayed into the source and the solvent droplets are charged. The solvent of the droplets gradually evaporates and gas-phase ions are released. The ionisation takes place under atmospheric pressure.	APCI Unselect MALDI Unselect CI Unselect ESI Unselect EI Unselect
Sample solution is sprayed into the source and the solvent is evaporated. The gas phase molecules are ionised with the help of corona discharge needle. The ionisation takes place under atmospheric pressure.	APCI Unselect MALDI Unselect CI Unselect ESI Unselect EI Unselect
Sample is mixed with matrix material solution, spotted on a target plate, and dried. The dried spot is irradiated with laser that evaporates and ionises the matrix material and sample components: the matrix material molecules are ionised by the laser and their charge is transferred to the sample molecules.	APCI Unselect MALDI Unselect CI Unselect ESI Unselect EI Unselect

Complete the sentence.

is responsible for ionising the sample molecules.

separates the ions according to their m/z value.

registers the created ions.

Course introduction
1. Overview of materials and techniques
2. Optical microscopy
3. Vibrational spectroscopy
- 3.1. IR Spectroscopy
  - * SELF TEST 3.1
- 3.2. Raman spectroscopy
  - * SELF TEST 3.2
4. Elemental and X-Ray methods
5. General aspects of chromatography and mass spectrometry
- 5.1. Chromatography
  - * SELF TEST 5.1
- 5.2. Mass spectrometry
  - * SELF TEST 5.2
6. Practical aspects of chromatography and mass spectrometry
- 6.1. Chromatography
  - * SELF TEST 6.1
- 6.2. Mass spectrometry
  - * SELF TEST 6.2
7. Multimethod analysis
Glossary
Acknowledgements
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