MOOC: Instrumental analysis of cultural heritage objects

SELF TEST 2

When analysing the surface of a painting in a museum, which type of microscope would be more reasonable to use?

Why is glycerine used for fibre identification with optical microscope? (There can be one or more correct answers) 

A paint is examined first under light microscope and then under fluorescence microscope to find out whether zinc white and starch are in the paint’s composition. Which of the following statements is accurate? (There can be one or more correct answers) 

Which type of microscopy is described?

Is often used as an in situ method; its most important part is a set of lenses; samples can be analysed with transmitted light and reflected light; can be used for the analysis of the layered structure of the paint sample
Fluorescence microscopy

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Light microscopy

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Polarized light microscopy

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High degree of sensitivity; can be used for quantitative and qualitative analysis; allows observing samples with optically anisotropic characters; paint components have different appearances
Fluorescence microscopy

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Light microscopy

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Polarized light microscopy

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Needs excitation source and filters; is often integrated with other microscopes; is usually done in dark environment; can give preliminary information about dyes without sampling and extraction of dyes
Fluorescence microscopy

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Light microscopy

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Polarized light microscopy

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Which of the following statements applies to which type of fibre?

It has a wide round-shaped cross-section and fibre surface, which looks like it’s covered with scales
Wool

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Linen

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Cotton

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Polyamide

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It has a flat cross-section and apparent twist along with the fibre
Wool

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Linen

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Cotton

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Polyamide

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It has nodes along with the fibre
Wool

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Linen

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Cotton

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Polyamide

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It has a smoother surface, is shiny and resembles plastic wire
Wool

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Linen

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Cotton

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Polyamide

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