Artiklid – Lehekülg 2 – Ribolab

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A mutation in the ribosomal protein uS12 reveals novel functions of its universally conserved PNSA loop

Datta M, Pillai M, Modak MJ, Liiv A, Khaja FT, Hussain T, Remme J, Varshney U. The ribosomal protein uS12 is conserved across all domains of life. Recently, a heterozygous spontaneous mutation in human uS12 (corresponding to R49K mutation immediately ...

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21.06.2021

Luciferase-based reporter system for in vitro evaluation of elongation rate and processivity of ribosomes

Kisly I, Kattel C, Remme J, Tamm T. The elongation step of translation is a key contributor to the abundance, folding and quality of proteins and to the stability of mRNA. However, control over translation elongation has not been thoroughly ...

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04.06.2021

Pseudouridines of tRNA Anticodon Stem-Loop Have Unexpected Role in Mutagenesis in Pseudomonas sp

Tagel M, Ilves H, Leppik M, Jürgenstein K, Remme J, Kivisaar M. Pseudouridines are known to be important for optimal translation. In this study we demonstrate an unexpected link between pseudouridylation of tRNA and mutation frequency in Pseudomonas species. We observed that ...

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23.12.2020

Phenotypic effects of paralogous ribosomal proteins bL31A and bL31B in E. coli

Lilleorg S, Reier K, Volõnkin P, Remme J, Liiv A. Ribosomes are essential macromolecular complexes conducting protein biosynthesis in all domains of life. Cells can have heterogeneous ribosomes, i.e. ribosomes with various ribosomal RNA and ribosomal protein (r-protein) composition. However, ...

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15.07.2020

Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly

Huang S, Aleksashin NA, Loveland AB, Klepacki D, Reier K, Kefi A, Szal T, Remme J, Jaeger L, Vázquez-Laslop N, Korostelev AA, Mankin AS. 5S rRNA is an indispensable component of cytoplasmic ribosomes in all species. The functions of 5S ...

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09.06.2020

Functional Interactions of Ribosomal Intersubunit Bridges in Saccharomyces cerevisiae

Tamm T, Kisly I, Remme J. Ribosomes of Archaea and Eukarya share higher homology with each other than with bacterial ribosomes. For example, there is a set of 35 r-proteins that are specific only for archaeal and eukaryotic ribosomes. Three ...

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01.12.2019

Assembly and functionality of the ribosome with tethered subunits

Aleksashin NA, Leppik M, Hockenberry AJ, Klepacki D, Vázquez-Laslop N, Jewett MC, Remme J, Mankin AS. Ribo-T is an engineered ribosome whose small and large subunits are tethered together by linking 16S rRNA and 23S rRNA in a single molecule. ...

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25.02.2019

Pseudomonas putida Responds to the Toxin GraT by Inducing Ribosome Biogenesis Factors and Repressing TCA Cycle Enzymes

Ainelo A, Porosk R, Kilk K, Rosendahl S, Remme J, Hõrak R. The potentially self-poisonous toxin-antitoxin modules are widespread in bacterial chromosomes, but despite extensive studies, their biological importance remains poorly understood. Here, we used whole-cell proteomics to study the ...

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09.02.2019

Ribosomal protein eL24, involved in two intersubunit bridges, stimulates translation initiation and elongation

Kisly I, Remme J, Tamm T. Interactions between subunits in the Saccharomyces cerevisiae ribosome are mediated by universal and eukaryote-specific intersubunit bridges. Universal bridges are positioned close to the ribosomal functional centers, while eukaryote-specific bridges are mainly located on the ...

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10.01.2019

Bacterial ribosome heterogeneity: Changes in ribosomal protein composition during transition into stationary growth phase

Lilleorg S, Reier K, Pulk A, Liiv A, Tammsalu T, Peil L, Cate JHD, Remme J. Ribosomes consist of many small proteins and few large RNA molecules. Both components are necessary for ribosome functioning during translation. According to widely accepted ...

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01.01.2019