Cell culture and RNA

We offer a number of services in molecular and cell biology and can help with experimental design, data analysis and interpretation.

miRNA

• miRNA target search and pathway analysis
• miRNA RT-qPCR
• Cloning and verification of miRNA targets in the Luciferase assay
• Analysis of miRNA influence on endogenous targets
• miRNA in situ hybridization

RNA

• total RNA isolation
• RT-qPCR

Cell culture

• Human primary keratinocytes, melanocytes and fibroblasts
• Human reconstituted epidermis
• Bronchial epithelial cells
• PBMCs
• Human monocytes and monocyte derived dendritic cells and macrophages
• Primary T cells
• Human cell-lines (HeLa, HEK293, BEAS-2B, HaCat, A549, THP-1 and PPC-1)

Molecular and cell biology

• Transfection of cell-lines, primary cells and T cells
• Proliferation and apoptosis assays
• Cloning
• Immunofluorescence analysis
• Western blot analysis
• Flow cytometry and cell sorting

Data analysis and consultation

• Statistical data analysis using R
• Transcriptome analysis
• Visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets with „Imaris“ platform
• Research publishing

Additional information http://www.biomeditsiin.ut.ee/en/departments/rna-biology-research-group-0