{"id":31,"date":"2025-11-03T14:25:51","date_gmt":"2025-11-03T12:25:51","guid":{"rendered":"https:\/\/sisu.ut.ee\/sime\/?page_id=31"},"modified":"2025-11-03T14:25:52","modified_gmt":"2025-11-03T12:25:52","slug":"cell-culture-and-rna","status":"publish","type":"page","link":"https:\/\/sisu.ut.ee\/sime\/cell-culture-and-rna\/","title":{"rendered":"Cell culture and RNA"},"content":{"rendered":"<p>We offer a number of services in molecular and cell biology and can help with experimental design, data analysis and interpretation.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\">miRNA<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>miRNA target search and pathway analysis<\/li>\n\n\n\n<li>miRNA RT-qPCR<\/li>\n\n\n\n<li>Cloning and verification of miRNA targets in the Luciferase assay<\/li>\n\n\n\n<li>Analysis of miRNA influence on endogenous targets<\/li>\n\n\n\n<li>miRNA in situ hybridization<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">RNA<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>total RNA isolation<\/li>\n\n\n\n<li>RT-qPCR<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Cell culture<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Human primary keratinocytes, melanocytes and fibroblasts<\/li>\n\n\n\n<li>Human reconstituted epidermis<\/li>\n\n\n\n<li>Bronchial epithelial cells<\/li>\n\n\n\n<li>PBMCs<\/li>\n\n\n\n<li>Human monocytes and monocyte derived dendritic cells and macrophages<\/li>\n\n\n\n<li>Primary T cells<\/li>\n\n\n\n<li>Human cell-lines (HeLa, HEK293, BEAS-2B, HaCat, A549, THP-1 and PPC-1)<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Molecular and cell biology<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Transfection of cell-lines, primary cells and T cells<\/li>\n\n\n\n<li>Proliferation and apoptosis assays<\/li>\n\n\n\n<li>Cloning<\/li>\n\n\n\n<li>Immunofluorescence analysis<\/li>\n\n\n\n<li>Western blot analysis<\/li>\n\n\n\n<li>Flow cytometry and cell sorting<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">Data analysis and consultation<\/h2>\n\n\n\n<ul class=\"wp-block-list\">\n<li>Statistical data analysis using R<\/li>\n\n\n\n<li>Transcriptome analysis<\/li>\n\n\n\n<li>Visualization, analysis, segmentation and interpretation of 3D and 4D microscopy datasets with \u201eImaris\u201c platform<\/li>\n\n\n\n<li>Research publishing<\/li>\n<\/ul>\n\n\n\n<p>Additional information\u00a0<a href=\"http:\/\/www.biomeditsiin.ut.ee\/en\/departments\/rna-biology-research-group-0\">http:\/\/www.biomeditsiin.ut.ee\/en\/departments\/rna-biology-research-group-0<\/a><\/p>\n\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>We offer a number of services in molecular and cell biology and can help with experimental design, data analysis and interpretation. miRNA RNA Cell culture Molecular and cell biology Data analysis and consultation Additional information\u00a0http:\/\/www.biomeditsiin.ut.ee\/en\/departments\/rna-biology-research-group-0<\/p>\n","protected":false},"author":280,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"class_list":["post-31","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sisu.ut.ee\/sime\/wp-json\/wp\/v2\/pages\/31","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sisu.ut.ee\/sime\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sisu.ut.ee\/sime\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/sime\/wp-json\/wp\/v2\/users\/280"}],"replies":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/sime\/wp-json\/wp\/v2\/comments?post=31"}],"version-history":[{"count":1,"href":"https:\/\/sisu.ut.ee\/sime\/wp-json\/wp\/v2\/pages\/31\/revisions"}],"predecessor-version":[{"id":33,"href":"https:\/\/sisu.ut.ee\/sime\/wp-json\/wp\/v2\/pages\/31\/revisions\/33"}],"wp:attachment":[{"href":"https:\/\/sisu.ut.ee\/sime\/wp-json\/wp\/v2\/media?parent=31"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}