{"id":1256,"date":"2019-02-25T12:00:00","date_gmt":"2019-02-25T10:00:00","guid":{"rendered":"https:\/\/sisu.ut.ee\/ribolab\/?p=1256"},"modified":"2024-10-23T11:47:22","modified_gmt":"2024-10-23T08:47:22","slug":"assembly-and-functionality-of-the-ribosome-with-tethered-subunits","status":"publish","type":"post","link":"https:\/\/sisu.ut.ee\/ribolab\/assembly-and-functionality-of-the-ribosome-with-tethered-subunits\/","title":{"rendered":"Assembly and functionality of the ribosome with tethered subunits"},"content":{"rendered":"<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<p>Aleksashin NA, Leppik M, Hockenberry AJ, Klepacki D, V\u00e1zquez-Laslop N, Jewett MC, Remme J, Mankin AS.<\/p>\n\n\n\n<p>Ribo-T is an engineered ribosome whose small and large subunits are tethered together by linking 16S rRNA and 23S rRNA in a single molecule. Although Ribo-T can support cell proliferation in the absence of wild type ribosomes, Ribo-T cells grow slower than those with wild type ribosomes. Here, we show that cell growth defect is likely explained primarily by slow Ribo-T assembly rather than its imperfect functionality. Ribo-T maturation is stalled at a late assembly stage. Several post-transcriptional rRNA modifications and some ribosomal proteins are underrepresented in the accumulated assembly intermediates and rRNA ends are incompletely trimmed. Ribosome profiling of Ribo-T cells shows no defects in translation elongation but reveals somewhat higher occupancy by Ribo-T of the start codons and to a lesser extent stop codons, suggesting that subunit tethering mildly affects the initiation and termination stages of translation. Understanding limitations of Ribo-T system offers ways for its future development.<\/p>\n\n\n\n<p>Nat Commun. 2019 Feb 25;10(1):930. doi: <a href=\"https:\/\/www.nature.com\/articles\/s41467-019-08892-w\" data-type=\"link\" data-id=\"https:\/\/www.nature.com\/articles\/s41467-019-08892-w\" target=\"_blank\" rel=\"noreferrer noopener\">10.1038\/s41467-019-08892-w.<\/a><br><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30804338\/\" data-type=\"link\" data-id=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/30804338\/\" target=\"_blank\" rel=\"noreferrer noopener\">Pubmed<\/a><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Aleksashin NA, Leppik M, Hockenberry AJ, Klepacki D, V\u00e1zquez-Laslop N, Jewett MC, Remme J, Mankin AS. Ribo-T is an engineered ribosome whose small and large subunits are tethered together by linking 16S rRNA and 23S rRNA in a single molecule. &#8230;<\/p>\n","protected":false},"author":194,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[11],"tags":[],"class_list":["post-1256","post","type-post","status-publish","format-standard","hentry","category-artiklid"],"acf":[],"_links":{"self":[{"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/posts\/1256","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/users\/194"}],"replies":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/comments?post=1256"}],"version-history":[{"count":1,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/posts\/1256\/revisions"}],"predecessor-version":[{"id":1257,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/posts\/1256\/revisions\/1257"}],"wp:attachment":[{"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/media?parent=1256"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/categories?post=1256"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/tags?post=1256"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}