{"id":1215,"date":"2019-12-01T12:00:00","date_gmt":"2019-12-01T10:00:00","guid":{"rendered":"https:\/\/sisu.ut.ee\/ribolab\/?p=1215"},"modified":"2024-10-23T11:31:07","modified_gmt":"2024-10-23T08:31:07","slug":"functional-interactions-of-ribosomal-intersubunit-bridges-in-saccharomyces-cerevisiae","status":"publish","type":"post","link":"https:\/\/sisu.ut.ee\/ribolab\/functional-interactions-of-ribosomal-intersubunit-bridges-in-saccharomyces-cerevisiae\/","title":{"rendered":"Functional Interactions of Ribosomal Intersubunit Bridges in\u00a0Saccharomyces cerevisiae"},"content":{"rendered":"<div class=\"wp-block-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<p>Tamm T, Kisly I, Remme J.<\/p>\n\n\n\n<p>Ribosomes of Archaea and Eukarya share higher homology with each other than with bacterial ribosomes. For example, there is a set of 35 r-proteins that are specific only for archaeal and eukaryotic ribosomes. Three of these proteins-eL19, eL24, and eL41-participate in interactions between ribosomal subunits. The eukaryote-specific extensions of r-proteins eL19 and eL24 form two intersubunit bridges eB12 and eB13, which are present only in eukaryotic ribosomes. The third r-protein, eL41, forms bridge eB14. Notably, eL41 is found in all eukaryotes but only in some Archaea. It has been shown that bridges eB12 and eB13 are needed for efficient translation, while r-protein eL41 plays a minor role in ribosome function. Here, the functional interactions between intersubunit bridges were studied using budding yeast strains lacking different combinations of the abovementioned bridges\/proteins. The growth phenotypes, levels of\u00a0<em>in vivo<\/em>\u00a0translation, ribosome-polysome profiles, and\u00a0<em>in vitro<\/em>\u00a0association of ribosomal subunits were analyzed. The results show a genetic interaction between r-protein eL41 and the eB12 bridge-forming region of eL19, and between r-proteins eL41 and eL24. It was possible to construct viable yeast strains with Archaea-like ribosomes lacking two or three eukaryote-specific bridges. These strains display slow growth and a poor translation phenotype. In addition, bridges eB12 and eB13 appear to cooperate during ribosome subunit association. These results indicate that nonessential structural elements of r-proteins become highly important in the context of disturbed subunit interactions. Therefore, eukaryote-specific bridges may contribute to the evolutionary success of eukaryotic translation machinery.<\/p>\n\n\n\n<p>Genetics. 2019 Dec;213(4):1329-1339. doi: <a href=\"https:\/\/academic.oup.com\/genetics\/article\/213\/4\/1329\/5930639\" data-type=\"link\" data-id=\"https:\/\/academic.oup.com\/genetics\/article\/213\/4\/1329\/5930639\" target=\"_blank\" rel=\"noreferrer noopener\">10.1534\/genetics.119.302777.<\/a><br><a href=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31649153\/\" data-type=\"link\" data-id=\"https:\/\/pubmed.ncbi.nlm.nih.gov\/31649153\/\" target=\"_blank\" rel=\"noreferrer noopener\">Pubmed<\/a><\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Tamm T, Kisly I, Remme J. Ribosomes of Archaea and Eukarya share higher homology with each other than with bacterial ribosomes. For example, there is a set of 35 r-proteins that are specific only for archaeal and eukaryotic ribosomes. Three &#8230;<\/p>\n","protected":false},"author":194,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"categories":[11],"tags":[],"class_list":["post-1215","post","type-post","status-publish","format-standard","hentry","category-artiklid"],"acf":[],"_links":{"self":[{"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/posts\/1215","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/users\/194"}],"replies":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/comments?post=1215"}],"version-history":[{"count":2,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/posts\/1215\/revisions"}],"predecessor-version":[{"id":1237,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/posts\/1215\/revisions\/1237"}],"wp:attachment":[{"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/media?parent=1215"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/categories?post=1215"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/sisu.ut.ee\/ribolab\/wp-json\/wp\/v2\/tags?post=1215"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}