\n| \n Matrix effect in broad sense (sample matrix source)<\/p>\n<\/td>\n | \n High<\/p>\n<\/td>\n | \n 6 different sources<\/p>\n<\/td>\n | \n Can be assessed under selectivity studies. Influences trueness\u00a0(recovery and ionization suppression), LoQ\/LoD<\/p>\n<\/td>\n<\/tr>\n |
\n| \n Mass spectrometry<\/strong><\/p>\n<\/td>\n<\/tr>\n\n| \n Drying gas temp<\/p>\n<\/td>\n | \n Low<\/p>\n<\/td>\n | \n \u00b1 10 \u00b0C<\/p>\n<\/td>\n | \n Drying gas temperature can influence the analyte ionization efficiency in the ion source<\/p>\n<\/td>\n<\/tr>\n | \n| \n Nebulizer gas pressure\/flow rate<\/p>\n<\/td>\n | \n Low<\/p>\n<\/td>\n | \n \u00b1 5 psi \/<\/p>\n \u00b1 1 L\/min<\/p>\n<\/td>\n | \n Nebulizer gas pressure\/flow rate can influence the analyte ionization efficiency in the ion source<\/p>\n<\/td>\n<\/tr>\n | \n| \n Ion source configuration (Nebulizer position)<\/p>\n<\/td>\n | \n High (if configurations can vary)<\/p>\n Not applicable (if fixed source)<\/p>\n<\/td>\n | \n According to the ion source design. Should be varied if the source is used in different configurations.<\/p>\n<\/td>\n | \n Ion source configuration can influence the spray and ionization efficiency in the ion source<\/p>\n \u00a0<\/p>\n<\/td>\n<\/tr>\n | \n| \n Ion source condition (Nebulizer aging, ion source contamination)<\/p>\n<\/td>\n | \n High<\/p>\n<\/td>\n | \n After analysis of samples versus cleaned system<\/p>\n<\/td>\n | \n Contamination can spontaneously accumulate when analyzing a series of samples.<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n \u00a0<\/div>\n ***<\/div>\n (1)\u00a0This list is not exhaustive, there can be different other parameters that, depending on the situation, could be changed.\u00a0<\/div>\n (2)\u00a0This grading is generic, corresponding to most common situations. In specific cases the grading can be different.\u00a0<\/div>\n (3)\u00a0These extents of parameter variation can be considered as possible defaults. In practice different extents can be used, but they should be realistic.\u00a0<\/div>\n (4) In the case of UHPLC the smaller changes in the injection solvent have stronger influence, especially if larger injection volume is used.<\/div>\n \u00a0<\/p>\n\n\n \n 10.1_robustness_and_ruggedness_relation_to_lc-ms_method_development.pdf<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"Evaluation of ruggedness\u00a0and\u00a0robustness\u00a0is very important in the case of LC-MS, as there are large number of system parameters, some of which are difficult to control. As a result, some of the key performance characteristics are also difficult to control or …<\/p>\n","protected":false},"author":60,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"class_list":["post-8","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/pages\/8","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/users\/60"}],"replies":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/comments?post=8"}],"version-history":[{"count":4,"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/pages\/8\/revisions"}],"predecessor-version":[{"id":1566,"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/pages\/8\/revisions\/1566"}],"wp:attachment":[{"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/media?parent=8"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}} | |