{"id":35,"date":"2024-04-04T00:39:47","date_gmt":"2024-04-03T21:39:47","guid":{"rendered":"https:\/\/sisu.ut.ee\/lcms_method_validation\/34-sensitivity\/"},"modified":"2024-04-16T15:19:43","modified_gmt":"2024-04-16T12:19:43","slug":"34-sensitivity","status":"publish","type":"page","link":"https:\/\/sisu.ut.ee\/lcms_method_validation\/34-sensitivity\/","title":{"rendered":"3.4. Sensitivity"},"content":{"rendered":"<h5 style=\"text-align: center;\"><div class=\"ratio ratio-16x9 mb-3\"><div class=\"video-placeholder-wrapper video-placeholder-wrapper--16x9\">\n\t\t\t    <div class=\"video-placeholder d-flex justify-content-center align-items-center\">\n\t\t\t        <div class=\"overlay text-white p-2 w-100 text-center d-block justify-content-center align-items-center\">\n\t\t\t            <div>To view third-party content, please accept cookies.<\/div>\n\t\t\t            <button class=\"btn btn-secondary btn-sm mt-1 consent-change\">Change consent<\/button>\n\t\t\t        <\/div>\n\t\t\t    <\/div>\n\t\t\t<\/div>\n<\/div>Sensitivity<\/h5>\n<h5 style=\"text-align: center;\"><a href=\"http:\/\/www.uttv.ee\/naita?id=23289\" target=\"_blank\" rel=\"noopener\">http:\/\/www.uttv.ee\/naita?id=23289<\/a><\/h5>\n<h5 style=\"text-align: center;\"><a href=\"https:\/\/www.youtube.com\/watch?v=h59-5voDcQU\" target=\"_blank\" rel=\"noopener\">https:\/\/www.youtube.com\/watch?v=h59-5voDcQU<\/a><\/h5>\n<h5 style=\"text-align: center;\"><\/h5>\n<p>Another parameter concerning the relationship between the analyte signal and the concentration\u00a0is sensitivity. <strong>Sensitivity is the change in the response of a measuring instrument divided by corresponding change in the stimulus or simply the gradient of the calibration function<\/strong>\u00a0<a href=\"#\" data-bs-toggle=\"modal\" data-bs-target=\"#popup-modal\" data-title=\"(1)\" data-content=\"(1). Sensitivity is often interpreted as related to the detection\/determination ability. For example, in the recent FDA\u2019s Bioanalytical Method Validation guidance document, a\u00a0sensitivity is defined as \u201cthe lowest analyte concentration that can be measured with acceptable accuracy and precision (i.e., LLoQ)\u201d. In this course the term sensitivity is not used in this meaning. \">(1)<\/a>\u00a0The numerical value (and unit) of a sensitivity is arbitrary and depends on the instrument used and its settings.\u00a0Sensitivity is not an essential parameter during a method validation.\u00a0However,\u00a0it is very important in the\u00a0method optimization and quality assurance procedures for routine monitoring of the instrument\u2019s performance. Sensitivity of a method can be easily estimated from the linearity evaluation experiments.<\/p>\n<p>Although sensitivity is not to be confused with the limit of quantitation (LoQ), these terms are interrelated: with a given signal to noise ratio, the higher the sensitivity, the lower the LoD and LoQ. Sensitivity is also directly related to the ionization suppression \u2013 in fact the essence of an ionization suppression is a decrease of sensitivity due to the\u00a0co-eluting compounds (see <a href=\"https:\/\/sisu.ut.ee\/lcms_method_validation\/5-trueness\/\">chapter 5<\/a>).<\/p>\n<p>The main use of a sensitivity as a parameter is threefold:<\/p>\n<ol>\n<li>an optimization (for maximizing sensitivity) of the method parameters during a method development,<\/li>\n<li>a daily optimization of the instrument parameters and<\/li>\n<li>monitoring of the instrument performance.<\/li>\n<\/ol>\n<p>***<\/p>\n<p>(1) Sensitivity is often interpreted as related to the detection\/determination ability. For example, in the recent FDA\u2019s Bioanalytical Method Validation guidance document, a sensitivity is defined as \u201cthe lowest analyte concentration that can be measured with acceptable accuracy and precision (i.e., LLoQ)\u201d. In this course the term sensitivity is not used in this meaning.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>To view third-party content, please accept cookies. Change consent Sensitivity http:\/\/www.uttv.ee\/naita?id=23289 https:\/\/www.youtube.com\/watch?v=h59-5voDcQU Another parameter concerning the relationship between the analyte signal and the concentration\u00a0is sensitivity. Sensitivity is the change in the response of a measuring instrument divided by corresponding change &#8230;<\/p>\n","protected":false},"author":60,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"class_list":["post-35","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/pages\/35","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/users\/60"}],"replies":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/comments?post=35"}],"version-history":[{"count":2,"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/pages\/35\/revisions"}],"predecessor-version":[{"id":1256,"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/pages\/35\/revisions\/1256"}],"wp:attachment":[{"href":"https:\/\/sisu.ut.ee\/lcms_method_validation\/wp-json\/wp\/v2\/media?parent=35"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}