{"id":1192,"date":"2024-04-11T14:43:44","date_gmt":"2024-04-11T11:43:44","guid":{"rendered":"https:\/\/sisu.ut.ee\/lcms_method_validation\/self-test-10-1\/"},"modified":"2024-04-11T14:43:44","modified_gmt":"2024-04-11T11:43:44","slug":"self-test-10-1","status":"publish","type":"page","link":"https:\/\/sisu.ut.ee\/lcms_method_validation\/self-test-10-1\/","title":{"rendered":"Self test 10.1"},"content":{"rendered":"<div id=\"watupro_quiz\" class=\"quiz-area single-page-quiz\">\n<p id=\"submittingExam25\" style=\"display:none;text-align:center;\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sisu.ut.ee\/wp-content\/plugins\/watupro\/img\/loading.gif\" width=\"16\" height=\"16\"><\/p>\n\n\n\n<form action=\"\" method=\"post\" class=\"quiz-form\" id=\"quiz-25\" enctype=\"multipart\/form-data\">\n<div class=\"watu-question \" id=\"question-1\" style=\";\"><div id=\"questionWrap-1\" class=\"   watupro-question-id-102\">\n\t\t\t<div class=\"question-content\"><div><span class=\"watupro_num\">1. <\/span>Look at the list of compounds. Please indicate the compounds with increasing retention time in reversed phase stationary phase (C18) when the mobile phase pH is changed one unit from pH 10 to 11.<br>\r\n<table style=\"height: 216px; width: 30%; border: 1px solid #000000; margin: 5px;\" border=\"1\" cellspacing=\"1\"><tbody><tr style=\"height: 24px;\"><td style=\"border: 1px solid #000000; height: 24px; width: 61.7706%; padding: 5px; vertical-align: middle;\" width=\"64\"><strong>Compound<\/strong><\/td><td style=\"border: 1px solid #000000; height: 24px; width: 37.2233%; padding: 5px; vertical-align: middle;\" width=\"64\"><strong>pKa<\/strong><\/td><\/tr><tr style=\"height: 24px;\"><td style=\"border: 1px solid #000000; vertical-align: middle; height: 24px; text-align: center; width: 98.9939%;\" colspan=\"2\"><strong>Bases<\/strong><\/td><\/tr><tr style=\"height: 24px;\"><td style=\"border: 1px solid #000000; height: 24px; width: 61.7706%; padding: 5px; vertical-align: middle;\">Pyrrolidine<\/td><td style=\"border: 1px solid #000000; height: 24px; width: 37.2233%; padding: 5px; vertical-align: middle;\">11.27<\/td><\/tr><tr style=\"height: 24px;\"><td style=\"border: 1px solid #000000; height: 24px; width: 61.7706%; padding: 5px; vertical-align: middle;\">Piperidine<\/td><td style=\"border: 1px solid #000000; height: 24px; width: 37.2233%; padding: 5px; vertical-align: middle;\">11.12<\/td><\/tr><tr style=\"height: 24px;\"><td style=\"border: 1px solid #000000; height: 24px; width: 61.7706%; padding: 5px; vertical-align: middle;\">2-methylpyridine<\/td><td style=\"border: 1px solid #000000; height: 24px; width: 37.2233%; padding: 5px; vertical-align: middle;\">5.97<\/td><\/tr><tr style=\"height: 24px;\"><td style=\"border: 1px solid #000000; vertical-align: middle; height: 24px; text-align: center; width: 98.9939%;\" colspan=\"2\"><strong>Acids<\/strong><\/td><\/tr><tr style=\"height: 24px;\"><td style=\"border: 1px solid #000000; height: 24px; width: 61.7706%; padding: 5px; vertical-align: middle;\">2-nitrophenol<\/td><td style=\"border: 1px solid #000000; height: 24px; width: 37.2233%; padding: 5px; vertical-align: middle;\">7.17<\/td><\/tr><tr style=\"height: 24px;\"><td style=\"border: 1px solid #000000; height: 24px; width: 61.7706%; padding: 5px; vertical-align: middle;\">2,5-dinitrophenol<\/td><td style=\"border: 1px solid #000000; height: 24px; width: 37.2233%; padding: 5px; vertical-align: middle;\">5.15<\/td><\/tr><tr style=\"height: 24px;\"><td style=\"border: 1px solid #000000; height: 24px; width: 61.7706%; padding: 5px; vertical-align: middle;\">3-nitroaniline<\/td><td style=\"border: 1px solid #000000; height: 24px; width: 37.2233%; padding: 5px; vertical-align: middle;\">17.9<\/td><\/tr><\/tbody><\/table><\/div><input type=\"hidden\" name=\"question_id[]\" id=\"qID_1\" value=\"102\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType102\" class=\"answerTypeCnt1\" value=\"checkbox\"><!-- end question-content--><\/div><div class=\"question-choices watupro-choices-columns \" id=\"questionChoices102\"><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-102[]\" id=\"answer-id-258\" class=\"answer   answerof-102 \" value=\"258\"><label for=\"answer-id-258\" id=\"answer-label-258\" class=\" answer\"><span><p>Pyrrolidine<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-102[]\" id=\"answer-id-259\" class=\"answer   answerof-102 \" value=\"259\"><label for=\"answer-id-259\" id=\"answer-label-259\" class=\" answer\"><span><p>Piperidine<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-102[]\" id=\"answer-id-260\" class=\"answer   answerof-102 \" value=\"260\"><label for=\"answer-id-260\" id=\"answer-label-260\" class=\" answer\"><span><p>2-methylpyridine<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-102[]\" id=\"answer-id-261\" class=\"answer   answerof-102 \" value=\"261\"><label for=\"answer-id-261\" id=\"answer-label-261\" class=\" answer\"><span><p>2-nitrophenol<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-102[]\" id=\"answer-id-262\" class=\"answer   answerof-102 \" value=\"262\"><label for=\"answer-id-262\" id=\"answer-label-262\" class=\" answer\"><span><p>2,5-dinitrophenol<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-102[]\" id=\"answer-id-263\" class=\"answer   answerof-102 \" value=\"263\"><label for=\"answer-id-263\" id=\"answer-label-263\" class=\" answer\"><span><p>3-nitroaniline<\/p><\/span><\/label><\/div><!-- end question-choices--><\/div><!-- end questionWrap--><\/div>\t\t\t\t\t<div style=\"display:none;\" id=\"liveResult-1\">\t\t   \n\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sisu.ut.ee\/wp-content\/plugins\/watupro\/img\/loading.gif\" width=\"16\" height=\"16\" alt=\"Loading...\" title=\"Loading...\">\u00a0Loading...\t\t\t\t\t<\/div>\t\n\t\t\t<\/div> \n\t   \t<p id=\"liveResBtn-102\" style=\"clear:both;\"><input type=\"button\" id=\"liveResultBtn102\" class=\"liveResultBtn1 watupro-live-result-btn\" value=\"See Answer\" onclick=\"WatuPRO.liveResult(102, 1);\"><\/p>\n\t   <div class=\"watu-question \" id=\"question-2\" style=\";\"><div id=\"questionWrap-2\" class=\"   watupro-question-id-103\">\n\t\t\t<div class=\"question-content\"><div><span class=\"watupro_num\">2. <\/span>Indicate correct statements\u00a0concerning \u00a0influence of the changes in liquid chromatography.<br>\r\n<br>\r\n\u00a0<\/div><input type=\"hidden\" name=\"question_id[]\" id=\"qID_2\" value=\"103\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType103\" class=\"answerTypeCnt2\" value=\"checkbox\"><!-- end question-content--><\/div><div class=\"question-choices watupro-choices-columns \" id=\"questionChoices103\"><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-103[]\" id=\"answer-id-267\" class=\"answer   answerof-103 \" value=\"267\"><label for=\"answer-id-267\" id=\"answer-label-267\" class=\" answer\"><span><p>Changes in eluent flow rate should be excluded in robustness tests since my LC-MS method is saved with fixed flow rate and therefore I do not need to evaluate the influence of this parameter.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-103[]\" id=\"answer-id-266\" class=\"answer   answerof-103 \" value=\"266\"><label for=\"answer-id-266\" id=\"answer-label-266\" class=\" answer\"><span><p>Column batch and age can influence the retention time, resolution and possibly therefore also ionization.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-103[]\" id=\"answer-id-264\" class=\"answer   answerof-103 \" value=\"264\"><label for=\"answer-id-264\" id=\"answer-label-264\" class=\" answer\"><span><p>Changes in pH have a high impact on method performance in if analyte\u2019s p<em>K<\/em><sub>a<\/sub> value is very close to the eluent pH.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-103[]\" id=\"answer-id-265\" class=\"answer   answerof-103 \" value=\"265\"><label for=\"answer-id-265\" id=\"answer-label-265\" class=\" answer\"><span><p>Changes in the salts and ion pair reagents concentrations do not have an effect to the method performance.<\/p><\/span><\/label><\/div><!-- end question-choices--><\/div><!-- end questionWrap--><\/div>\t\t\t\t\t<div style=\"display:none;\" id=\"liveResult-2\">\t\t   \n\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sisu.ut.ee\/wp-content\/plugins\/watupro\/img\/loading.gif\" width=\"16\" height=\"16\" alt=\"Loading...\" title=\"Loading...\">\u00a0Loading...\t\t\t\t\t<\/div>\t\n\t\t\t<\/div> \n\t   \t<p id=\"liveResBtn-103\" style=\"clear:both;\"><input type=\"button\" id=\"liveResultBtn103\" class=\"liveResultBtn2 watupro-live-result-btn\" value=\"See Answer\" onclick=\"WatuPRO.liveResult(103, 2);\"><\/p>\n\t   <div class=\"watu-question \" id=\"question-3\" style=\";\"><div id=\"questionWrap-3\" class=\"   watupro-question-id-104\">\n\t\t\t<div class=\"question-content\"><div><span class=\"watupro_num\">3. <\/span>Indicate correct statements\u00a0concerning \u00a0influence\u00a0of the changes in sample preparation.<\/div><input type=\"hidden\" name=\"question_id[]\" id=\"qID_3\" value=\"104\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType104\" class=\"answerTypeCnt3\" value=\"checkbox\"><!-- end question-content--><\/div><div class=\"question-choices watupro-choices-columns \" id=\"questionChoices104\"><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-104[]\" id=\"answer-id-269\" class=\"answer   answerof-104 \" value=\"269\"><label for=\"answer-id-269\" id=\"answer-label-269\" class=\" answer\"><span><p>Injection solvent composition (the solvent used in the last step of sample preparation) do not influence my method\u2019s performance, since I use the same solvent composition each and every time.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-104[]\" id=\"answer-id-270\" class=\"answer   answerof-104 \" value=\"270\"><label for=\"answer-id-270\" id=\"answer-label-270\" class=\" answer\"><span><p>Different matrices do not influence my method\u2019s performance if I have evaluated matrix effects for my method.;<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-104[]\" id=\"answer-id-268\" class=\"answer   answerof-104 \" value=\"268\"><label for=\"answer-id-268\" id=\"answer-label-268\" class=\" answer\"><span><p>Extraction time, amount of solvent used during the extraction and also the composition of the solvents in the sample preparation procedure influences recovery and LoQ\/LoD.<\/p><\/span><\/label><\/div><!-- end question-choices--><\/div><!-- end questionWrap--><\/div>\t\t\t\t\t<div style=\"display:none;\" id=\"liveResult-3\">\t\t   \n\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sisu.ut.ee\/wp-content\/plugins\/watupro\/img\/loading.gif\" width=\"16\" height=\"16\" alt=\"Loading...\" title=\"Loading...\">\u00a0Loading...\t\t\t\t\t<\/div>\t\n\t\t\t<\/div> \n\t   \t<p id=\"liveResBtn-104\" style=\"clear:both;\"><input type=\"button\" id=\"liveResultBtn104\" class=\"liveResultBtn3 watupro-live-result-btn\" value=\"See Answer\" onclick=\"WatuPRO.liveResult(104, 3);\"><\/p>\n\t   <div class=\"watu-question \" id=\"question-4\" style=\";\"><div id=\"questionWrap-4\" class=\"   watupro-question-id-105\">\n\t\t\t<div class=\"question-content\"><div><span class=\"watupro_num\">4. <\/span>Indicate correct statements\u00a0concerning \u00a0influence\u00a0of the changes in mass spectrometry.<\/div><input type=\"hidden\" name=\"question_id[]\" id=\"qID_4\" value=\"105\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType105\" class=\"answerTypeCnt4\" value=\"checkbox\"><!-- end question-content--><\/div><div class=\"question-choices watupro-choices-columns \" id=\"questionChoices105\"><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-105[]\" id=\"answer-id-273\" class=\"answer   answerof-105 \" value=\"273\"><label for=\"answer-id-273\" id=\"answer-label-273\" class=\" answer\"><span><p>Ion source configuration can influence spray and ionization efficiency in the ion source.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-105[]\" id=\"answer-id-274\" class=\"answer   answerof-105 \" value=\"274\"><label for=\"answer-id-274\" id=\"answer-label-274\" class=\" answer\"><span><p>Drying gas temperature does not influence my method performance.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-105[]\" id=\"answer-id-271\" class=\"answer   answerof-105 \" value=\"271\"><label for=\"answer-id-271\" id=\"answer-label-271\" class=\" answer\"><span><p>Changes in the ion source cleanliness can affect method performance during the analytical run.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-105[]\" id=\"answer-id-275\" class=\"answer   answerof-105 \" value=\"275\"><label for=\"answer-id-275\" id=\"answer-label-275\" class=\" answer\"><span><p>Nebulizer gas pressure\/flow rate can influence analyte ionization efficiency in the ion source.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-105[]\" id=\"answer-id-272\" class=\"answer   answerof-105 \" value=\"272\"><label for=\"answer-id-272\" id=\"answer-label-272\" class=\" answer\"><span><p>Evaluation of the changes in the ion source configuration is not needed if the source is fixed.<\/p><\/span><\/label><\/div><!-- end question-choices--><\/div><!-- end questionWrap--><\/div>\t\t\t\t\t<div style=\"display:none;\" id=\"liveResult-4\">\t\t   \n\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sisu.ut.ee\/wp-content\/plugins\/watupro\/img\/loading.gif\" width=\"16\" height=\"16\" alt=\"Loading...\" title=\"Loading...\">\u00a0Loading...\t\t\t\t\t<\/div>\t\n\t\t\t<\/div> \n\t   \t<p id=\"liveResBtn-105\" style=\"clear:both;\"><input type=\"button\" id=\"liveResultBtn105\" class=\"liveResultBtn4 watupro-live-result-btn\" value=\"See Answer\" onclick=\"WatuPRO.liveResult(105, 4);\"><\/p>\n\t   <div class=\"watu-question \" id=\"question-5\" style=\";\"><div id=\"questionWrap-5\" class=\"   watupro-question-id-106\">\n\t\t\t<div class=\"question-content\"><p><span class=\"watupro_num\">5. <\/span>Indicate the correct influence (<strong>Low, Medium, High<\/strong>) of the parameter to the method performance:<\/p>\n<p style=\"padding-left: 30px;\">1. Liquid chromatography<\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td valign=\"top\"><strong>Parameter<\/strong><\/td>\n<td valign=\"top\"><strong>Change in the parameter<\/strong><\/td>\n<td valign=\"top\"><strong>Influence<\/strong><\/td>\n<\/tr>\n<tr>\n<td valign=\"top\">pH<\/td>\n<td valign=\"top\">- 1 unit<\/td>\n<td valign=\"top\"><select name=\"gap_106_1\" class=\"watupro-gap answer answerof-106\"><option value=\"\"><\/option><option value=\"High\">High<\/option><option value=\"Medium\">Medium<\/option><option value=\"Low\">Low<\/option><\/select><\/td>\n<\/tr>\n<tr>\n<td valign=\"top\">Organic solvent<\/td>\n<td valign=\"top\">+ 10%<\/td>\n<td valign=\"top\"><select name=\"gap_106_2\" class=\"watupro-gap answer answerof-106\"><option value=\"\"><\/option><option value=\"High\">High<\/option><option value=\"Low\">Low<\/option><option value=\"Medium\">Medium<\/option><\/select><\/td>\n<\/tr>\n<tr>\n<td valign=\"top\">Column temperature<\/td>\n<td valign=\"top\">+15 \u00b0C<\/td>\n<td valign=\"top\"><select name=\"gap_106_3\" class=\"watupro-gap answer answerof-106\"><option value=\"\"><\/option><option value=\"High\">High<\/option><option value=\"Medium\">Medium<\/option><option value=\"Low\">Low<\/option><\/select><\/td>\n<\/tr>\n<tr>\n<td valign=\"top\">Eluent flow rate<\/td>\n<td valign=\"top\">+ 20%<\/td>\n<td valign=\"top\"><select name=\"gap_106_4\" class=\"watupro-gap answer answerof-106\"><option value=\"\"><\/option><option value=\"Low\">Low<\/option><option value=\"High\">High<\/option><option value=\"Medium\">Medium<\/option><\/select><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>\u00a0<\/p>\n<p style=\"padding-left: 30px;\">2. Mass spectrometry<\/p>\n<table border=\"1\" cellspacing=\"0\" cellpadding=\"0\">\n<tbody>\n<tr>\n<td valign=\"top\"><strong>Parameter<\/strong><\/td>\n<td valign=\"top\"><strong>Change in the parameter<\/strong><\/td>\n<td valign=\"top\"><strong>Influence<\/strong><\/td>\n<\/tr>\n<tr>\n<td valign=\"top\">Drying gas temp<\/td>\n<td valign=\"top\">+ 10 \u00b0C<\/td>\n<td valign=\"top\"><select name=\"gap_106_5\" class=\"watupro-gap answer answerof-106\"><option value=\"\"><\/option><option value=\"High\">High<\/option><option value=\"Medium\">Medium<\/option><option value=\"Low\">Low<\/option><\/select><\/td>\n<\/tr>\n<tr>\n<td valign=\"top\">Nebulizer gas pressure\/flow rate<\/td>\n<td valign=\"top\">+ 5 psi \/ + 1 L\/min<\/td>\n<td valign=\"top\"><select name=\"gap_106_6\" class=\"watupro-gap answer answerof-106\"><option value=\"\"><\/option><option value=\"Medium\">Medium<\/option><option value=\"High\">High<\/option><option value=\"Low\">Low<\/option><\/select><\/td>\n<\/tr>\n<tr>\n<td valign=\"top\">Ion source configuration (Nebulizer position)<\/td>\n<td valign=\"top\">Variations in the configuration during the last usage<\/td>\n<td valign=\"top\"><select name=\"gap_106_7\" class=\"watupro-gap answer answerof-106\"><option value=\"\"><\/option><option value=\"High\">High<\/option><option value=\"Low\">Low<\/option><option value=\"Medium\">Medium<\/option><\/select><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<input type=\"hidden\" name=\"question_id[]\" id=\"qID_5\" value=\"106\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType106\" class=\"answerTypeCnt5\" value=\"gaps\"><!-- end question-content--><\/div><!-- end questionWrap--><\/div>\t\t\t\t\t<div style=\"display:none;\" id=\"liveResult-5\">\t\t   \n\t\t\t\t\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sisu.ut.ee\/wp-content\/plugins\/watupro\/img\/loading.gif\" width=\"16\" height=\"16\" alt=\"Loading...\" title=\"Loading...\">\u00a0Loading...\t\t\t\t\t<\/div>\t\n\t\t\t<\/div> \n\t   \t<p id=\"liveResBtn-106\" style=\"clear:both;\"><input type=\"button\" id=\"liveResultBtn106\" class=\"liveResultBtn5 watupro-live-result-btn\" value=\"See Answer\" onclick=\"WatuPRO.liveResult(106, 5);\"><\/p>\n\t   <div style=\"display:none\" id=\"question-6\">\n\t<div class=\"question-content\">\n\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sisu.ut.ee\/wp-content\/plugins\/watupro\/img\/loading.gif\" width=\"16\" height=\"16\" alt=\"Loading...\" title=\"Loading...\">\u00a0Loading...\t<\/div>\n<\/div>\n\n<br>\n\t\n\t\t\t<div class=\"watupro_buttons flex \" id=\"watuPROButtons25\">\n\t\t  <div id=\"prev-question\" style=\"display:none;\"><input type=\"button\" value=\"&lt; 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