{"id":1162,"date":"2024-04-11T14:18:24","date_gmt":"2024-04-11T11:18:24","guid":{"rendered":"https:\/\/sisu.ut.ee\/lcms_method_validation\/self-test-5-4b\/"},"modified":"2024-04-11T14:18:24","modified_gmt":"2024-04-11T11:18:24","slug":"self-test-5-4b","status":"publish","type":"page","link":"https:\/\/sisu.ut.ee\/lcms_method_validation\/self-test-5-4b\/","title":{"rendered":"Self-test 5.4B"},"content":{"rendered":"
\n

<\/p>\n\n\n\n

\n
\n\t\t\t

1. <\/span>Matrix effect can be calculated both in the signal scale and in the concentration scale. Below signals for post-extraction spiked apple extract and a calibration graph is given. The apple extract was fortified with thiabendazole at 0.05 mg\/kg level.<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\n

c (mg\/kg)<\/strong><\/p>\n<\/td>\n

Signal<\/strong><\/td>\n<\/tr>\n
0.001<\/td>\n525357<\/td>\n<\/tr>\n
0.001<\/td>\n519754<\/td>\n<\/tr>\n
0.001<\/td>\n576415<\/td>\n<\/tr>\n
0.005<\/td>\n1516738<\/td>\n<\/tr>\n
0.01<\/td>\n2353920<\/td>\n<\/tr>\n
0.01<\/td>\n2311093<\/td>\n<\/tr>\n
0.01<\/td>\n2343350<\/td>\n<\/tr>\n
0.05<\/td>\n9986609<\/td>\n<\/tr>\n
0.1<\/td>\n20330928<\/td>\n<\/tr>\n
0.25<\/td>\n44231346<\/td>\n<\/tr>\n
0.5<\/td>\n87330954<\/td>\n<\/tr>\n
Sample<\/td>\n8490871<\/td>\n<\/tr>\n
Sample<\/td>\n8337484<\/td>\n<\/tr>\n
Sample<\/td>\n\n

9159663<\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u00a0<\/p>\n

Q1. Calculate the matrix effect from the signals.<\/p>\n

Please give the answer in percents with one decimal place and please use dot as decimals separator (i.e. please give 91.7% and not 0.917; 91,7% or 91.765%).
\n<\/p>\n

Q2. Calculate the matrix effect from the concentrations.<\/p>\n

Please give the answer in percents with one decimal place and please use dot as decimals separator (i.e. please give 91.7% and not 0.917; 91,7% or 91.765%).
\n<\/p>\n

Q3. Compare the results of Q1 and Q2 and explain why the results are somewhat different?<\/p>\n

\n\t\t\t
\n\t\t\t
\n\t\t\t
To view third-party content, please accept cookies.<\/div>\n\t\t\t