{"id":1142,"date":"2024-04-11T13:53:50","date_gmt":"2024-04-11T10:53:50","guid":{"rendered":"https:\/\/sisu.ut.ee\/lcms_method_validation\/self-test-2-7\/"},"modified":"2024-04-11T13:53:50","modified_gmt":"2024-04-11T10:53:50","slug":"self-test-2-7","status":"publish","type":"page","link":"https:\/\/sisu.ut.ee\/lcms_method_validation\/self-test-2-7\/","title":{"rendered":"Self test 2.7"},"content":{"rendered":"
\n

<\/p>\n\n\n\n

\n
\n\t\t\t
1. <\/span>LC with MS detection is perhaps the most reliable means for confirmation of analyte\u2018s identity. Please rank the following identity confirmation situations according to reliability (1 is the most reliable). The European Commission implementing regulation 2021\/808 can be used as guidance.
LC retention time (k > 1) and a single MS\/MS transition<\/td>\n\t\t\t\t\t\t
1

Unselect<\/p><\/div>

4

Unselect<\/p><\/div>

2

Unselect<\/p><\/div>

3

Unselect<\/p><\/div><\/div><\/div><\/td><\/tr>

LC retention time on two different columns (both have k > 1) and a single MS\/MS transition (same transitions for both columns). <\/td>\n\t\t\t\t\t\t
1

Unselect<\/p><\/div>

4

Unselect<\/p><\/div>

2

Unselect<\/p><\/div>

3

Unselect<\/p><\/div><\/div><\/div><\/td><\/tr>

LC retention time (k > 1) and MS\/MS with a single precursor ion with two product ions<\/td>\n\t\t\t\t\t\t
1

Unselect<\/p><\/div>

4

Unselect<\/p><\/div>

2

Unselect<\/p><\/div>

3

Unselect<\/p><\/div><\/div><\/div><\/td><\/tr>

LC retention time (k > 1), MS\/MS with two transitions, of which one of the product ions is detected with HRMS<\/td>\n\t\t\t\t\t\t
1

Unselect<\/p><\/div>

4

Unselect<\/p><\/div>

2

Unselect<\/p><\/div>

3

Unselect<\/p><\/div><\/div><\/div><\/td><\/tr><\/table><\/div><\/div><\/div>\t\t\t\t\t

\t\t \n\t\t\t\t\t\t\"Loading...\"\u00a0Loading...\t\t\t\t\t<\/div>\t\n\t\t\t<\/div> \n\t \t

<\/p>\n\t

\n\t\t\t
2. <\/span>HPLC analysis of one analyte in one sample and 5 calibration solutions was carried out.
\r\n
\r\nAssess if the analyte presence in the sample can be confirmed by the retention time criterion set by SANTE.
\r\n
Analysis<\/td>Ret. Time of analyte, min<\/td><\/tr>
Calibrant 1<\/td>4.298<\/td><\/tr>
Calibrant 2<\/td>4.413<\/td><\/tr>
Calibrant 3<\/td>4.211<\/td><\/tr>
Calibrant 4<\/td>4.368<\/td><\/tr>
Calibrant 5<\/td>4.183<\/td><\/tr>
Sample<\/td>4.122<\/td><\/tr><\/tbody><\/table><\/div><\/div>