{"id":704,"date":"2024-04-11T01:48:01","date_gmt":"2024-04-10T22:48:01","guid":{"rendered":"https:\/\/sisu.ut.ee\/heritage-analysis\/self-test-6-2\/"},"modified":"2024-07-18T14:32:28","modified_gmt":"2024-07-18T11:32:28","slug":"self-test-6-2","status":"publish","type":"page","link":"https:\/\/sisu.ut.ee\/heritage-analysis\/self-test-6-2\/","title":{"rendered":"SELF TEST 6.2"},"content":{"rendered":"<div id=\"watupro_quiz\" class=\"quiz-area single-page-quiz\">\n<p id=\"submittingExam15\" style=\"display:none;text-align:center;\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sisu.ut.ee\/wp-content\/plugins\/watupro\/img\/loading.gif\" width=\"16\" height=\"16\"><\/p>\n\n<div class=\"watupro-exam-description\" id=\"description-quiz-15\"><h2><span style=\"color: #000000;\">Questions about practical aspects of <\/span><span style=\"color: #b22222;\"><strong>mass spectrometry<\/strong><\/span>.<\/h2>\n<p>\u00a0<\/p>\n<\/div>\n\n<form action=\"\" method=\"post\" class=\"quiz-form\" id=\"quiz-15\" enctype=\"multipart\/form-data\">\n<div class=\"watu-question \" id=\"question-1\" style=\";\"><div id=\"questionWrap-1\" class=\"  watupro-required-105 watupro-question-id-105\">\n\t\t\t<div class=\"question-content\"><p>Are the following statement true or false?\u00a0<\/p>\n<p><select name=\"gap_105_1\" class=\"watupro-gap answer answerof-105\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 MALDI-MS is suitable for the analysis of small and large molecules|such as compounds present in dyes|<\/p>\n<p style=\"margin-left: 80px;\">\u00a0oils|waxes|resins|proteins|saccharides|synthetic\u00a0polymers etc.\u00a0<\/p>\n<p><select name=\"gap_105_2\" class=\"watupro-gap answer answerof-105\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 ESI-MS is suitable for the analysis of small and large molecules|such as compounds present in dyes|oils|<\/p>\n<p style=\"margin-left: 80px;\">\u00a0waxes|resins|proteins|saccharides|synthetic polymers etc.\u00a0<\/p>\n<p><select name=\"gap_105_3\" class=\"watupro-gap answer answerof-105\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 APCI-MS is suitable for the analysis of large molecules|such as compounds present in dyes|oils|waxes|<\/p>\n<p style=\"margin-left: 80px;\">\u00a0and resins.<\/p>\n<p><select name=\"gap_105_4\" class=\"watupro-gap answer answerof-105\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 CI-MS is suitable for the analysis of large molecules|such as compounds present in dyes|resins|polymers.<\/p>\n<p><select name=\"gap_105_5\" class=\"watupro-gap answer answerof-105\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 EI-MS is suitable for the analysis of small and large molecules|such as compounds present in dyes|oils|<\/p>\n<p style=\"margin-left: 80px;\">\u00a0waxes|and resins.\u00a0<\/p>\n<input type=\"hidden\" name=\"question_id[]\" id=\"qID_1\" value=\"105\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType105\" class=\"answerTypeCnt1\" value=\"gaps\"><input type=\"hidden\" id=\"watupro-required-question-105\"><!-- end question-content--><\/div><!-- end questionWrap--><\/div><\/div><div class=\"watu-question \" id=\"question-2\" style=\";\"><div id=\"questionWrap-2\" class=\"  watupro-required-106 watupro-question-id-106\">\n\t\t\t<div class=\"question-content\"><div><p>What to consider for selecting the suitable matrix material for MALDI-MS analysis?<span style=\"font-size:12px;\"> (There can be one or more correct answers)\u00a0<\/span><\/p><\/div><input type=\"hidden\" name=\"question_id[]\" id=\"qID_2\" value=\"106\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType106\" class=\"answerTypeCnt2\" value=\"checkbox\"><input type=\"hidden\" id=\"watupro-required-question-106\"><!-- end question-content--><\/div><div class=\"question-choices watupro-choices-columns \" id=\"questionChoices106\"><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-106[]\" id=\"answer-id-470\" class=\"answer   answerof-106 \" value=\"470\"><label for=\"answer-id-470\" id=\"answer-label-470\" class=\" answer\"><span><p>Matrix material must absorb at the laser wavelength that is used.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-106[]\" id=\"answer-id-471\" class=\"answer   answerof-106 \" value=\"471\"><label for=\"answer-id-471\" id=\"answer-label-471\" class=\" answer\"><span><p>Selection of matrix material depends on the analysed sample (lipide, resin, saccharide, etc.).<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-106[]\" id=\"answer-id-472\" class=\"answer   answerof-106 \" value=\"472\"><label for=\"answer-id-472\" id=\"answer-label-472\" class=\" answer\"><span><p>Basic matrix material mixed with the basic sample is more suitable for the analysis in positive ion mode, and acidic matrix material mixed with the sample that possesses acidic compounds is more suitable for negative ion mode.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-106[]\" id=\"answer-id-473\" class=\"answer   answerof-106 \" value=\"473\"><label for=\"answer-id-473\" id=\"answer-label-473\" class=\" answer\"><span><p>Solvent(s) used for the sample and matrix material do not have to be compatible with each other.<\/p><\/span><\/label><\/div><!-- end question-choices--><\/div><!-- end questionWrap--><\/div><\/div><div class=\"watu-question \" id=\"question-3\" style=\";\"><div id=\"questionWrap-3\" class=\"  watupro-required-107 watupro-question-id-107\">\n\t\t\t<div class=\"question-content\"><div><p>Please choose the correct statement(s) that apply for direct MS analysis. <span style=\"font-size:12px;\">(There can be one or more correct answers)\u00a0<\/span><\/p><\/div><input type=\"hidden\" name=\"question_id[]\" id=\"qID_3\" value=\"107\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType107\" class=\"answerTypeCnt3\" value=\"checkbox\"><input type=\"hidden\" id=\"watupro-required-question-107\"><!-- end question-content--><\/div><div class=\"question-choices watupro-choices-columns \" id=\"questionChoices107\"><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-107[]\" id=\"answer-id-474\" class=\"answer   answerof-107 \" value=\"474\"><label for=\"answer-id-474\" id=\"answer-label-474\" class=\" answer\"><span><p>With direct MS it is possibility to use smaller sample amounts compared to sample size needed for LC-MS or GC-MS analysis.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-107[]\" id=\"answer-id-475\" class=\"answer   answerof-107 \" value=\"475\"><label for=\"answer-id-475\" id=\"answer-label-475\" class=\" answer\"><span><p>Sample preparation for direct MS analysis has more steps than usual LC-MS or GC-MS sample preparation.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-107[]\" id=\"answer-id-476\" class=\"answer   answerof-107 \" value=\"476\"><label for=\"answer-id-476\" id=\"answer-label-476\" class=\" answer\"><span><p>The selection of solvents suitable for direct MS analysis is bigger than for LC-MS and GC-MS analysis.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-107[]\" id=\"answer-id-477\" class=\"answer   answerof-107 \" value=\"477\"><label for=\"answer-id-477\" id=\"answer-label-477\" class=\" answer\"><span><p>For direct MS analysis the sample must always be derivatised.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-107[]\" id=\"answer-id-478\" class=\"answer   answerof-107 \" value=\"478\"><label for=\"answer-id-478\" id=\"answer-label-478\" class=\" answer\"><span><p>For direct MS analysis the sample components must be ionised.<\/p><\/span><\/label><\/div><!-- end question-choices--><\/div><!-- end questionWrap--><\/div><\/div><div class=\"watu-question \" id=\"question-4\" style=\";\"><div id=\"questionWrap-4\" class=\"  watupro-required-108 watupro-question-id-108\">\n\t\t\t<div class=\"question-content\"><div><p>Which of the following statements are true? <span style=\"font-size:12px;\">(There can be one or more correct answers)\u00a0<\/span><\/p><\/div><input type=\"hidden\" name=\"question_id[]\" id=\"qID_4\" value=\"108\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType108\" class=\"answerTypeCnt4\" value=\"checkbox\"><input type=\"hidden\" id=\"watupro-required-question-108\"><!-- end question-content--><\/div><div class=\"question-choices watupro-choices-columns \" id=\"questionChoices108\"><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-108[]\" id=\"answer-id-479\" class=\"answer   answerof-108 \" value=\"479\"><label for=\"answer-id-479\" id=\"answer-label-479\" class=\" answer\"><span><p>For MALDI-MS analysis it is necessary that the sample is fully dissolved.\u00a0<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-108[]\" id=\"answer-id-480\" class=\"answer   answerof-108 \" value=\"480\"><label for=\"answer-id-480\" id=\"answer-label-480\" class=\" answer\"><span><p>For MALDI-MS analysis the sample must be co-crystallised with a suitable matrix material.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-108[]\" id=\"answer-id-481\" class=\"answer   answerof-108 \" value=\"481\"><label for=\"answer-id-481\" id=\"answer-label-481\" class=\" answer\"><span><p>With ESI-MS hazy sample solution can be analysed.\u00a0<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-108[]\" id=\"answer-id-482\" class=\"answer   answerof-108 \" value=\"482\"><label for=\"answer-id-482\" id=\"answer-label-482\" class=\" answer\"><span><p>With APCI-MS it is not possible to analyse samples without derivatisation.\u00a0<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-108[]\" id=\"answer-id-483\" class=\"answer   answerof-108 \" value=\"483\"><label for=\"answer-id-483\" id=\"answer-label-483\" class=\" answer\"><span><p>With ESI-MS only dissolved samples can be analysed.\u00a0<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-108[]\" id=\"answer-id-484\" class=\"answer   answerof-108 \" value=\"484\"><label for=\"answer-id-484\" id=\"answer-label-484\" class=\" answer\"><span><p>Identification of compounds from EI-MS spectra is easy because the obtained mass spectra are simple and contain mainly information about molecular ions.\u00a0<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-108[]\" id=\"answer-id-485\" class=\"answer   answerof-108 \" value=\"485\"><label for=\"answer-id-485\" id=\"answer-label-485\" class=\" answer\"><span><p>Identification of compounds from ESI-MS spectra is complicated due to high fragmentation.\u00a0<\/p><\/span><\/label><\/div><!-- end question-choices--><\/div><!-- end questionWrap--><\/div><\/div><div class=\"watu-question \" id=\"question-5\" style=\";\"><div id=\"questionWrap-5\" class=\"  watupro-required-109 watupro-question-id-109\">\n\t\t\t<div class=\"question-content\"><p>An paint sample from a 19th century painting is analysed with MALDI-MS to determine the binder (e.g. oil|wax|protein|etc.) used in the paint. Are the following statements true or false?<br><select name=\"gap_109_1\" class=\"watupro-gap answer answerof-109\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 The sample must be fully dissolved and sample solution must be filtered before analysis with MALDI-MS.\u00a0<\/p>\n<p><select name=\"gap_109_2\" class=\"watupro-gap answer answerof-109\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 The sample must be mixed with matrix material and co-crystallised with it for MALDI-MS analysis.<\/p>\n<p><select name=\"gap_109_3\" class=\"watupro-gap answer answerof-109\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 The sample compounds must be derivatised before the MALDI-MS analysis.<\/p>\n<p><select name=\"gap_109_4\" class=\"watupro-gap answer answerof-109\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 Very small sample (few \u03bcg) is sufficient for analysis.\u00a0<\/p>\n<p><select name=\"gap_109_5\" class=\"watupro-gap answer answerof-109\"><option value=\"\"><\/option><option value=\"True\">True<\/option><option value=\"False\">False<\/option><\/select>\u00a0 \u00a0 The macromolecules fragmented before analysis.\u00a0<\/p>\n<p><select name=\"gap_109_6\" class=\"watupro-gap answer answerof-109\"><option value=\"\"><\/option><option value=\"False\">False<\/option><option value=\"True\">True<\/option><\/select>\u00a0 \u00a0 The oil binder component can be analysed only in negative mode.\u00a0<\/p>\n<input type=\"hidden\" name=\"question_id[]\" id=\"qID_5\" value=\"109\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType109\" class=\"answerTypeCnt5\" value=\"gaps\"><input type=\"hidden\" id=\"watupro-required-question-109\"><!-- end question-content--><\/div><!-- end questionWrap--><\/div><\/div><div class=\"watu-question \" id=\"question-6\" style=\";\"><div id=\"questionWrap-6\" class=\"  watupro-required-110 watupro-question-id-110\">\n\t\t\t<div class=\"question-content\"><div><p>An original varnish sample from a 19th century painting is analysed with EI-MS. Which of the following statements are true?\u00a0<span style=\"font-size:12px;\">(There can be one or more correct answers)<\/span><\/p><\/div><input type=\"hidden\" name=\"question_id[]\" id=\"qID_6\" value=\"110\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType110\" class=\"answerTypeCnt6\" value=\"checkbox\"><input type=\"hidden\" id=\"watupro-required-question-110\"><!-- end question-content--><\/div><div class=\"question-choices watupro-choices-columns \" id=\"questionChoices110\"><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-110[]\" id=\"answer-id-487\" class=\"answer   answerof-110 \" value=\"487\"><label for=\"answer-id-487\" id=\"answer-label-487\" class=\" answer\"><span><p>The sample does not require any sample preparation when direct temperature EI-MS is used as analysis method.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-110[]\" id=\"answer-id-488\" class=\"answer   answerof-110 \" value=\"488\"><label for=\"answer-id-488\" id=\"answer-label-488\" class=\" answer\"><span><p>The varnish sample must be mixed with a matrix solution and co-crystallised with the matrix before EI-MS analysis.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-110[]\" id=\"answer-id-489\" class=\"answer   answerof-110 \" value=\"489\"><label for=\"answer-id-489\" id=\"answer-label-489\" class=\" answer\"><span><p>The varnish sample must be fully dissolved and derivatised before analysis with EI-MS coupled to a gas chromatograph.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-110[]\" id=\"answer-id-490\" class=\"answer   answerof-110 \" value=\"490\"><label for=\"answer-id-490\" id=\"answer-label-490\" class=\" answer\"><span><p>The amount of varnish sample needed for EI-MS analysis must at least be 1 mg.<\/p><\/span><\/label><\/div><!-- end question-choices--><\/div><!-- end questionWrap--><\/div><\/div><div class=\"watu-question \" id=\"question-7\" style=\";\"><div id=\"questionWrap-7\" class=\"  watupro-required-111 watupro-question-id-111\">\n\t\t\t<div class=\"question-content\"><div><p>A textile fibre sample from a 18th century tapestry is analysed with ESI-MS to determine the red dye used on the object. Which of the following statements are true? <span style=\"font-size:12px;\">(There can be one or more correct answers)\u00a0<\/span><\/p><\/div><input type=\"hidden\" name=\"question_id[]\" id=\"qID_7\" value=\"111\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType111\" class=\"answerTypeCnt7\" value=\"checkbox\"><input type=\"hidden\" id=\"watupro-required-question-111\"><!-- end question-content--><\/div><div class=\"question-choices watupro-choices-columns \" id=\"questionChoices111\"><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-111[]\" id=\"answer-id-491\" class=\"answer   answerof-111 \" value=\"491\"><label for=\"answer-id-491\" id=\"answer-label-491\" class=\" answer\"><span><p>The dye must be extracted from the fibre with a suitable solvent and the solution must be filtered (or centrifuged) for analysis with ESI-MS.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-111[]\" id=\"answer-id-492\" class=\"answer   answerof-111 \" value=\"492\"><label for=\"answer-id-492\" id=\"answer-label-492\" class=\" answer\"><span><p>The dyed textile fibre can be directly analysed with ESI-MS, no sample preparation is needed for analysis.\u00a0<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-111[]\" id=\"answer-id-493\" class=\"answer   answerof-111 \" value=\"493\"><label for=\"answer-id-493\" id=\"answer-label-493\" class=\" answer\"><span><p>The dyed fibre must be soaked with matrix material solution and co-crystallised with the matrix for ESI-MS analysis.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-111[]\" id=\"answer-id-494\" class=\"answer   answerof-111 \" value=\"494\"><label for=\"answer-id-494\" id=\"answer-label-494\" class=\" answer\"><span><p>Preferably more polar compounds in red dye are ionised.<\/p><\/span><\/label><\/div><div class=\"watupro-question-choice  \" dir=\"auto\"><input type=\"checkbox\" name=\"answer-111[]\" id=\"answer-id-495\" class=\"answer   answerof-111 \" value=\"495\"><label for=\"answer-id-495\" id=\"answer-label-495\" class=\" answer\"><span><p>The interpretation of the mass spectrum of dye is based on the retention times of the most intensive chromatographic peaks of the compounds, their <em>m\/z<\/em> values and isotope patterns.<\/p><\/span><\/label><\/div><!-- end question-choices--><\/div><!-- end questionWrap--><\/div><\/div><div class=\"watu-question \" id=\"question-8\" style=\";\"><div id=\"questionWrap-8\" class=\"  watupro-required-112 watupro-question-id-112\">\n\t\t\t<div class=\"question-content\"><p>Are following statements true?\u00a0<br><select name=\"gap_112_1\" class=\"watupro-gap answer answerof-112\"><option value=\"\"><\/option><option value=\"YES\">YES<\/option><option value=\"NO\">NO<\/option><\/select>\u00a0\u00a0\u00a0\u00a0It is possible to analyse dried polyvinyl acetate glue with APCI-MS.\u00a0<\/p>\n<p><select name=\"gap_112_2\" class=\"watupro-gap answer answerof-112\"><option value=\"\"><\/option><option value=\"NO\">NO<\/option><option value=\"YES\">YES<\/option><\/select>\u00a0\u00a0\u00a0\u00a0It is possible to analyse proteinous paint binder with MALDI-MS.<\/p>\n<p><select name=\"gap_112_3\" class=\"watupro-gap answer answerof-112\"><option value=\"\"><\/option><option value=\"NO\">NO<\/option><option value=\"YES\">YES<\/option><\/select>\u00a0 \u00a0 It is possible to analyse inorganic additives from the paint with EI-MS.<\/p>\n<p><select name=\"gap_112_4\" class=\"watupro-gap answer answerof-112\"><option value=\"\"><\/option><option value=\"YES\">YES<\/option><option value=\"NO\">NO<\/option><\/select>\u00a0\u00a0\u00a0\u00a0It is possible to analyse natural terpenoid resin from the varnish with ESI-MS.\u00a0<\/p>\n<p><select name=\"gap_112_5\" class=\"watupro-gap answer answerof-112\"><option value=\"\"><\/option><option value=\"NO\">NO<\/option><option value=\"YES\">YES<\/option><\/select>\u00a0\u00a0\u00a0\u00a0It is possible to analyse synthetic dyes with CI-MS.<\/p>\n<p>\u00a0<\/p>\n<input type=\"hidden\" name=\"question_id[]\" id=\"qID_8\" value=\"112\" class=\"watupro-question-id\"><input type=\"hidden\" id=\"answerType112\" class=\"answerTypeCnt8\" value=\"gaps\"><input type=\"hidden\" id=\"watupro-required-question-112\"><!-- end question-content--><\/div><!-- end questionWrap--><\/div><\/div><div style=\"display:none\" id=\"question-9\">\n\t<div class=\"question-content\">\n\t\t<img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/sisu.ut.ee\/wp-content\/plugins\/watupro\/img\/loading.gif\" width=\"16\" height=\"16\" alt=\"Loading...\" title=\"Loading...\">\u00a0Loading...\t<\/div>\n<\/div>\n\n<br>\n\t\n\t\t\t<div class=\"watupro_buttons flex \" id=\"watuPROButtons15\">\n\t\t  <div 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