{"id":34,"date":"2024-04-04T04:31:17","date_gmt":"2024-04-04T01:31:17","guid":{"rendered":"https:\/\/sisu.ut.ee\/heritage-analysis\/module2\/"},"modified":"2024-07-16T17:20:25","modified_gmt":"2024-07-16T14:20:25","slug":"module2","status":"publish","type":"page","link":"https:\/\/sisu.ut.ee\/heritage-analysis\/module2\/","title":{"rendered":"2. Optical microscopy"},"content":{"rendered":"<div style=\"height:30px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<p>In this course\u00a0the\u00a0general introduction to\u00a0optical microscopy and an overview of the different types of optical microscopes\u00a0 (stereo microscope, polarized light microscope, fluorescence microscope, etc.) will be given. Based on the type of materials (textile fibres, paint cross-sections, archaeological materials, etc.) practical examples and explanations will be given.<\/p>\n\n\n\n<h2 class=\"wp-block-heading\"><em><span style=\"color: #b22222;\">Microscopic analysis<\/span><\/em><\/h2>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<p><strong>Optical microscopy<\/strong> is often one of <strong>the first methods<\/strong> for the analysis of cultural heritage objects. It is a fast method for examing and identifying materials like <strong>fibres<\/strong>, <strong>paints<\/strong>, <strong>minerals<\/strong>, <strong>ceramics<\/strong>, <strong>metals<\/strong>, etc. This method is non-destructive for the sample, and in some cases, samples removing from the original object is not needed.<\/p>\n\n\n\n<p>Microscopes enable the magnification of small objects or smaller parts of the larger objects and provide visual information of the sample. It is possible to obtain valuable information about the structure, size, shape, colour, thickness, dimensions, and other characteristics of small features of the sample. Also, it allows to observe the surface of the artefacts without removing samples from the object and evaluate its condition (e.g. cracks, broken areas, moulds and dirt on the objects, etc.).\u00a0<\/p>\n\n\n\n<p>There are different types of microscopes available. They may differ by size, design, magnification range, resolution, etc. Nowadays, microscopes can be equipped with a CCD camera and are connected to the computer. Specific software allows to observe, take photos and manipulate the images of the sample. This gives more possibilities to examine and store photos of tiny objects. There are also small USB digital microscopes (so-called handheld digital microscopes) available, enabling the primary examination in situ in museums, churches, etc.<\/p>\n<\/div>\n\n\n\n<p><\/p>\n\n\n\n<p>In this lecture<strong> light microscopy<\/strong>,<strong> polarized light microscopy<\/strong>, and <strong>fluorescence microscopy<\/strong> are discussed.<\/p>\n\n\n\n<p><\/p><div class=\"ratio ratio-16x9 mb-3\"><div class=\"video-placeholder-wrapper video-placeholder-wrapper--16x9\">\n\t\t\t    <div class=\"video-placeholder d-flex justify-content-center align-items-center\">\n\t\t\t        <div class=\"overlay text-white p-2 w-100 text-center d-block justify-content-center align-items-center\">\n\t\t\t            <div>To view third-party content, please accept cookies.<\/div>\n\t\t\t            <button class=\"btn btn-secondary btn-sm mt-1 consent-change\">Change consent<\/button>\n\t\t\t        <\/div>\n\t\t\t    <\/div>\n\t\t\t<\/div>\n<\/div>\n\n\n\n<p>This section is divided into three subsections:<\/p>\n\n\n\n<ul class=\"wp-block-list\">\n<li><a title=\"\" href=\"https:\/\/sisu.ut.ee\/heritage-analysis\/21-light-microscopy\" data-url=\"https:\/\/sisu.ut.ee\/heritage-analysis\/21-light-microscopy\">2.1. Light microscopy<\/a><\/li>\n\n\n\n<li><a title=\"\" href=\"https:\/\/sisu.ut.ee\/heritage-analysis\/22-polarized-light-microscopy\" data-url=\"https:\/\/sisu.ut.ee\/heritage-analysis\/22-polarized-light-microscopy\">2.2. Polarized light microscopy<\/a><\/li>\n\n\n\n<li><a title=\"\" href=\"https:\/\/sisu.ut.ee\/heritage-analysis\/23-fluorescence-microscopy\" data-url=\"https:\/\/sisu.ut.ee\/heritage-analysis\/23-fluorescence-microscopy\">2.3. Fluorescence microscopy<\/a>\u00a0<\/li>\n<\/ul>\n\n\n\n<h2 class=\"wp-block-heading\">\u00a0<\/h2>\n\n\n\n<p>The slides used in the video can be downloaded from\u00a0here:<\/p>\n\n\n\n<div class=\"wp-block-group attached-files-group is-layout-constrained wp-block-group-is-layout-constrained\">\n<div class=\"wp-block-file\"><a id=\"wp-block-file--media-3417451b-f870-432c-aace-d6bd13396b24\" href=\"https:\/\/sisu.ut.ee\/wp-content\/uploads\/sites\/285\/microscopic_analysis.pdf\" target=\"_blank\" rel=\"noreferrer noopener\">Microscopic_analysis.pdf<\/a><\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>In this course\u00a0the\u00a0general introduction to\u00a0optical microscopy and an overview of the different types of optical microscopes\u00a0 (stereo microscope, polarized light microscope, fluorescence microscope, etc.) will be given. Based on the type of materials (textile fibres, paint cross-sections, archaeological materials, etc.) &#8230;<\/p>\n","protected":false},"author":151,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"class_list":["post-34","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sisu.ut.ee\/heritage-analysis\/wp-json\/wp\/v2\/pages\/34","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sisu.ut.ee\/heritage-analysis\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sisu.ut.ee\/heritage-analysis\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/heritage-analysis\/wp-json\/wp\/v2\/users\/151"}],"replies":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/heritage-analysis\/wp-json\/wp\/v2\/comments?post=34"}],"version-history":[{"count":3,"href":"https:\/\/sisu.ut.ee\/heritage-analysis\/wp-json\/wp\/v2\/pages\/34\/revisions"}],"predecessor-version":[{"id":784,"href":"https:\/\/sisu.ut.ee\/heritage-analysis\/wp-json\/wp\/v2\/pages\/34\/revisions\/784"}],"wp:attachment":[{"href":"https:\/\/sisu.ut.ee\/heritage-analysis\/wp-json\/wp\/v2\/media?parent=34"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}