{"id":17,"date":"2024-04-04T02:59:09","date_gmt":"2024-04-03T23:59:09","guid":{"rendered":"https:\/\/sisu.ut.ee\/gentransmed\/metabolomics-workgroup\/"},"modified":"2024-04-04T02:59:17","modified_gmt":"2024-04-03T23:59:17","slug":"metabolomics-workgroup","status":"publish","type":"page","link":"https:\/\/sisu.ut.ee\/gentransmed\/metabolomics-workgroup\/","title":{"rendered":"Metabolomics workgroup"},"content":{"rendered":"

Metabolomics<\/strong> is the qualitative and quantitative analysis of the human body\u2019s metabolism\u2019s low-molecular weight intermediates and end products to characterise the conditions\/diseases of an organism caused by metabolic changes and to find new biomarkers for early disease detection, monitoring and description over the course of treatment. The metabolomic approaches are designated for the analysis of low molecular weight compounds in biofluids and tissues. Metabolomic profiles (MP) provide an instantaneous snapshot of the entire physiology of a body, characterize functional individual differences in healthy population metabolomes and also help to discover novel potential disease-specific biomarkers for the diagnosis and follow-up of different pathological states. The MP are measured on a liquid chromatography-tandem mass spectrometer (LC-MS\/MS) or using a proton Nuclear Magnetic Resonance (1H NMR) approach. Two complementary appro\u00adaches\u2015untargeted and targeted metabolomic analysis\u2015are used to analyse differences in the MP of sample donors by supervised and unsupervised multivariate data mining methods such as principal component analysis, partial least square regression, hierarchical cluster analysis, heatmap displays, etc. The same methods can be used to filter out the individual compounds that cause the changes (e.g. novel markers of diseases) in MP.<\/p>\n

Our Metabolomics research group<\/strong> works in the core laboratory which was initiated by prof. M. Zilmer and prof. U. Soomets. The laboratory is currently lead by the prof. U. Soomets and senior research fellow K. Kilk. There are 5 PhD students working in the lab on a daily basis. We have both experience and competence to use high sensitive MS methods to provide investigation starting from untargeted and followed by targeted MP for large numbers of subjects at reasonable cost. Our new scientific workgroup was the first and so far the only in Estonia dedicated to metabolome analyses. The current bio- and translational medical integrated studies of Metabolomics research group are as follows:<\/p>\n