{"id":5,"date":"2024-04-04T03:43:07","date_gmt":"2024-04-04T00:43:07","guid":{"rendered":"https:\/\/sisu.ut.ee\/endothelialcentre\/researchandmethods\/"},"modified":"2024-04-22T14:34:47","modified_gmt":"2024-04-22T11:34:47","slug":"researchandmethods","status":"publish","type":"page","link":"https:\/\/sisu.ut.ee\/endothelialcentre\/researchandmethods\/","title":{"rendered":"RESEARCH &amp; METHODS"},"content":{"rendered":"<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\"><strong>Main Research Goals<\/strong><\/span><\/p>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">We aim to<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">describe structural and functional alterations of arteries in a wide range of clinical conditions<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">detect and affect interactions between arterial stiffness and hemodynamics<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">determine associations between arterial stiffness, endothelial dysfunction,\u00a0inflammation and oxidative stress<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">determine low-molecular-weight metabolites involved in the pathogenesis of arterial stiffness, endothelial dysfunction\u00a0and cardiovascular diseases using metabolomics approach<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">identify diagnostic and prognostic biomarkers of vascular damage and cardiovascular disease<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">test interventions intended to prevent vascular dysfunction and end-organ damage (e.g. antihypertensive drugs, remote ischemic preconditioning) in different clinical settings<\/span><\/li>\n<\/ul>\n<hr>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\"><strong style=\"color: #000000;\">Methods<\/strong><\/span><\/p>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Arterial stiffness and central hemodynamics<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Aortic pulse wave velocity (PWV) (<em>Sphygmocor XCEL\u00ae;<\/em>\u00a0AtCor Medical, Australia)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\"><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Pulse wave analysis (PWA) and central blood pressure (<em>Sphygmocor XCEL\u00ae;<\/em>\u00a0AtCor Medical,\u00a0Australia), (<em>Mobil-O-Graph\u00ae;<\/em>\u00a0I.E.M.,\u00a0Germany)<\/span><\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\"><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Small and large artery elasticity (<em>HDI\/PulseWave CR-2000 Research CardioVascular Profiling System<\/em>; Hypertension Diagnostics Inc., USA)<\/span><\/span><\/li>\n<\/ul>\n<hr>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Endothelial dysfunction, local arterial stiffness, carotid intima-media thickness<\/span><\/p>\n<ul>\n<li><span style=\"font-family: Arial,Helvetica,sans-serif;\"><span style=\"font-size: medium;\" data-mce-mark=\"1\">Vascular ultrasonography (<\/span><span style=\"color: #000000;\" data-mce-mark=\"1\"><span style=\"font-size: 16px;\"><em>Philips\u00a0CX-50;<\/em>\u00a0Philips Medical Systems Inc., USA;<\/span>\u00a0<\/span><span style=\"font-size: medium;\" data-mce-mark=\"1\"><em>M-Turbo<em>\u00ae<\/em>, Sonosite,\u00a0<\/em>USA)<\/span><\/span><\/li>\n<li><span style=\"color: #000000;\"><span style=\"font-family: Arial,Helvetica,sans-serif;\"><span style=\"font-size: 16px;\">Blood flow velocities\u00a0and flow volumes of arteries and veins<\/span><\/span><\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Flow-mediated dilation (FMD) (<em>FMD Studio, Cardiovascular Suite<\/em> <em>3.4<\/em>, Quipu, Italy)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Carotid artery stiffness (<em>Carotid Studio, Cardiovascular Suite 3.4, <\/em>Quipu, Italy)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Carotid intima-media thickness (<em>Carotid Studio, Cardiovascular Suite 3.4<\/em>, Quipu, Italy)<\/span><\/li>\n<\/ul>\n<hr>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Left ventricular hypertrophy, ankle-brachial index, volume plethysmography, claudication distance<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Echocardiography (<em>Philips\u00a0CX-50;<\/em>\u00a0Philips Medical Systems Inc., USA)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Electrocardiography (<em>MAC1200 ST;\u00a0<\/em><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">GE Healthcare, USA)<\/span><\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\"><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Air plethysmography (Dopplex ABility<em><em>\u00ae<\/em><\/em> ABI System, Huntleigh Healthcare; UK)<\/span><\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Doppler ultrasound (<em>The Portable ABI Kit;\u00a0<\/em>D.E. Hokanson, Inc.,\u00a0USA)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\"><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Treadmill<\/span> <span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">(<em>Vision Fitness T60;\u00a0<\/em>Johnson Health Tech. Co., Ltd.,\u00a0Taiwan)<\/span><\/span><\/li>\n<\/ul>\n<hr>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Cardiac autonomic function<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\"><span style=\"color: #000000; font-family: helvetica; font-size: medium;\">Heart rate variability analysis (<em>Kubios HRV Premium 3.1<\/em>, Finland)<\/span><\/span><\/li>\n<\/ul>\n<hr>\n<p><span style=\"font-family: helvetica; color: #000000; font-size: medium;\">Biomarkers<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Enzyme-linked immunosorbent assay (ELISA)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Semi-automated Biochip Array Technology analyzer (<em>The Evidence Investigator\u2122;<\/em>\u00a0Randox Laboratories, UK)<\/span><\/li>\n<\/ul>\n<hr>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Targeted and untargeted metabolomics (in cooperation with the Mass-Spectrometry Lab of the Department of Biochemistry)<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Tandem mass-spectrometry (MS\/MS)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Flow injection analysis-tandem mass spectrometry\u00a0(FIA-MS\/MS) +\u00a0targeted quantitative\u00a0metabolomics kits (<em>AbsoluteIDQ\u00ae p180 Kit;<\/em>\u00a0BIOCRATES Life Sciences AG, Austria)<\/span><\/li>\n<\/ul>\n<hr>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Animal models<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">In-vivo aortic stiffness and hemodynamic measurements in rats<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Histological analysis of aortic wall structure<\/span><\/li>\n<\/ul>\n<hr>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Other methods\/interventions<\/span><\/p>\n<ul>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Remote ischemic preconditioning<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Computer-tomography-based aortic calcification scoring system<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Coronary angiographic (SYNTAX, Gensini) and digital subtraction analysis-based scoring systems<\/span><\/li>\n<\/ul>\n<hr>\n<p><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">Statistical analysis in collaboration with professional statisticians<\/span><\/p>\n<ul>\n<li><em><span style=\"font-family: helvetica; font-size: medium; color: #000000;\" data-mce-mark=\"1\">R\u00a0<\/span><\/em><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">(R Development Core Team, Austria)<\/span><\/li>\n<li><span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\"><em>Stata<\/em> (Version 15.0; Stata Corporation., USA)<\/span><\/li>\n<li><em style=\"color: #000000; font-family: helvetica; font-size: medium;\">IBM SPSS Statistics<\/em> <span style=\"color: #000000; font-family: helvetica; font-size: medium;\" data-mce-mark=\"1\">(SPSS, USA)<\/span><\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>Main Research Goals We aim to describe structural and functional alterations of arteries in a wide range of clinical conditions detect and affect interactions between arterial stiffness and hemodynamics determine associations between arterial stiffness, endothelial dysfunction,\u00a0inflammation and oxidative stress determine &#8230;<\/p>\n","protected":false},"author":127,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"footnotes":""},"class_list":["post-5","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/sisu.ut.ee\/endothelialcentre\/wp-json\/wp\/v2\/pages\/5","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/sisu.ut.ee\/endothelialcentre\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/sisu.ut.ee\/endothelialcentre\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/endothelialcentre\/wp-json\/wp\/v2\/users\/127"}],"replies":[{"embeddable":true,"href":"https:\/\/sisu.ut.ee\/endothelialcentre\/wp-json\/wp\/v2\/comments?post=5"}],"version-history":[{"count":2,"href":"https:\/\/sisu.ut.ee\/endothelialcentre\/wp-json\/wp\/v2\/pages\/5\/revisions"}],"predecessor-version":[{"id":113,"href":"https:\/\/sisu.ut.ee\/endothelialcentre\/wp-json\/wp\/v2\/pages\/5\/revisions\/113"}],"wp:attachment":[{"href":"https:\/\/sisu.ut.ee\/endothelialcentre\/wp-json\/wp\/v2\/media?parent=5"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}